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ECG recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States)

Compound movements like squats, deadlifts, presses, rows, and pull-ups provide efficient stimulus

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Exercise and Physical Performance Research Age-Related Physical Decline Investigations in aging mice demonstrated that MOTS-c treatment significantly improved physical capacity across multiple age groups: Enhanced running endurance in young (2-month), middle-aged (12-month), and old (22-month) mice Improved grip strength and rotarod performance in aged animals Late-life intermittent treatment (beginning at 23.5 months) increased healthspan metrics Reversal of age-dependent decline in mitochondrial function Notably, human studies confirmed that exercise induces endogenous MOTS-c expression in both skeletal muscle tissue and circulation, suggesting evolutionary conservation of exercise-responsive signaling

96127 Brief emotional/behavioral assessment, per standardized instrument (e.g., PHQ9 for depression)