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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

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Thiamine, sometimes referred to as the anti-beriberi factor and neurine, is a key component of carbohydrate metabolism and is soluble in polar solvents such as water but insoluble in organic solvents

doi: 10.1016/j.cmet.2013.02.007

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