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Vitamin B12 can be converted to coenzyme B12 in tissues, and as such is essential for conversion of methylmalonate to succinate and synthesis of methionine from homocysteine, a reaction which also requires folate
This study used the NNMTi 5-amino-1-methylquinolinium (5A-M1Q), since our lab has set precedence using this probe molecule for proof-of-concept studies 8
This step helps prevent a vacuum from forming in the vial, making it easier to withdraw the medication
