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doi:10.1016/j.bbagen.2009.07.026

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This entire process is not a singular event but rather a stereoscopic, network-like cascade initiated by ischemia and amplified upon reperfusion (Figure 1)

Samples were centrifuged at 6000 rpm for 15 min at room temperature, plasma was divided into four Eppendorf tubes and kept at -80 until analysis

Y-27,632 is a competitive inhibitor of both Rho-associated protein kinase ROCK1 and ROCK2 (serine-threonine kinases) that competes with ATP in binding the catalytic binding sites of these kinases and thereby interferes with membrane protrusion and vesicle shedding through suppressed activity on LIM kinase and myosin light chain (MLC) 9