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Sonodynamic Therapy Low-intensity ultrasound can also be used to activate tissue that has been sensitized with a non-toxic chemical agent

Bacteria were harvested by centrifugation at 7,000 rpm 15 min and washed 10 times with a buffer containing (per liter) 8 g NaCl, 0.2 g KCl, and 0.24 g Na 2 HPO 4 (pH 7.4)

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Subsequently, 150 L of freshly prepared FRAP reagent (300 mM acetate buffer, 10 mM TPTZ, and 20 mM FeCl6 HO

Before the experiment began, all animals underwent a one-week acclimatization period to allow adaptation to the laboratory environment