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Overall, this study suggests that sleep deprivation plays a pivotal role in the pathological mechanism of depression and anxiety in PCOS mice by modulating tryptophan and lipid metabolism through the gut-brain axis.

Supplementation of 400 g/mL Trp in the media reversed the fungal growth with increased fungal dry weight (33.331.53 mg), and sclerotia weight (67.673.06 mg), highlighting Trp starvation as a key factor for reduced growth of the fungus

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Theories of depressive disorders include the monoaminergic, neurotrophic, glucocorticosteroid, glutaminergic, inflammatory, and cytokine theories