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1 2 Interdisciplinary Sciences: Computational Life Sciences

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Nrf2 activation directly upregulates the expression of -glutamylcysteine ligase (-GCL), the rate-limiting enzyme required for glutathione synthesis

Technical and biological hurdles are paramount, including the inefficient delivery of cells or vectors to widespread motor neurons across the central nervous system, host immune responses that can limit efficacy or durability, and unresolved long-term safety concerns such as off-target effects or uncontrolled cell differentiation (15, 60, 65)

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