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Each sample was centrifuged at 4000 rpm and separate plasma from RBCs

The long read lengths characteristic of third-generation sequencing technologies enable researchers to resolve complex genomic structures more effectively, particularly excelling in animal genome assembly

The relationship between the intron phase and sequence conservation at splice sites is associated with the evolution of spliceosomal introns ( PavGST classes, Subfamilies I, II, III, IV, and V exhibited peak conservation whereas subfamily VI, and VII showed a greater number of introns and mixed conservation at the splice site sequence (Figure 2)

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