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Many compounds that show promise in animal studies have not been thoroughly tested in humans

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After washing, cells were incubated with 50 l of crystal violet staining solution, for 20 min at room temperature, gently washed with distilled water and let air-drying for at least 2 h at room temperature

Research indicates that niacinamide formulations optimized at neutral pH may enhance efficacy in darker skin phototypes where enhanced permeation facilitates superior melanin transfer inhibition, making it particularly suitable for Indian dermatological practice where melasma prevalence ranges from 8-40% in certain populations

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