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An isoelectric point (pI) of 5.8, and net charges of +2.8 and 14.7 at pH 5.5 and pH 7.4, respectively, were calculated for the albumin variants (Supplementary Table 3)

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The second layer, the intestinal epithelium, consists of a single layer of several specialized epithelial cells, such as enterocytes, Goblet cells, Paneth cells, enteroendocrine cells, and microfold cells (Figure 1)

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