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10.1016/j.ejphar.2020.173288 87 TenrioM.GracilianoN

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To obtain the cytoplasm and cytosol, the hearts were washed in ice-cold homogenisation buffer (250 mM sucrose, 10 mM HEPES, and 1 mM EGTA, pH 7.4), homogenised in 2 ml buffer and centrifuged at 700 g for 10 min at 4 C to remove unbroken tissue and nuclei