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Description
Combinations of HRP-labeled secondary antibodies detected with DAB, DAB containing nickel chloride (DAB-Ni) or Nova Red reactions (Vector Laboratories), or alkaline phosphatase (AP) detected with BCIP reactions, were used to maximize contrast between the different antibodies

Decapitation in rats: latency to unconsciousness and the wave of death

Any research findings in this direction may be helpful for those with cachexia or sarcopenia

The absorbance was measured at 412 nm with a BioTek Synergy H1 hybrid Microplate Readers

Science 2009;325(5943):10171020