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Another relevant mechanism includes the disulfide-glutathione redox couple (GSH/GSSG) (Ursini and Maiorino, 2020)

LHON is one of the most common mitochondrial diseases and it involves a two-step process: an initial dysfunction of retinal ganglion cells, followed by a degeneration of their axons [96]

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Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes

Roy M, 2019, 'Feasibility of Silicon quantum dots to study dynamics of the tear film lipids', in INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE , ASSOC RESEARCH VISION OPHTHALMOLOGY INC, CANADA, Vancouver, Vol