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Working Concentrations Reported in the Literature For researchers designing in-vitro protocols, published concentration ranges provide a useful starting reference point (though any specific protocol should be validated against the relevant primary literature for the assay in question)

However, as your body naturally filters and excretes the vitamin, those stores gradually deplete

Balogh LM, Le Trong I, Kripps KA, Shireman LM, Stenkamp RE, Zhang W, et al

The interest towards this compound in the treatment of UTIs dates back to the 1970s when the first episodes of the emergence of antibiotic-resistant uropathogens prompted the use of alternative methods, when feasible, to antibiotic treatment [12,13]