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Western blot analyses For the in vitro studies, differentiated C2C12 cells were collected at the end of experiments by cell lysis in ice-cold RIPA buffer
Lastly, the International Restless Leg Syndrome Study Group rating scale (IRLSS) appraises the presence and severity of RLS and is a valuable tool specifically in patients with primary biliary cholangitis (31, 33, 36)
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