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The tissue lysates were centrifuged at 12,000 g for 15 min at 4 C

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In experimental models of myocardial ischemiareperfusion injury (during which the heart muscle is deprived of blood supply and then reperfused), damage to heart tissue is less severe in animals with sufficient zinc levels than in those with low zinc levels

Samples treated with MTC, on the other hand, show extensive lipoperoxidative damage, as shown by the very high cytoplasmic MDA concentration (Figure 4a, bar to the left)