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less commonly, IgA antibodies are the culprits

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Rx Only 2 mL Product does not contain plasma/albumin Single-dose only Takeda 6521276 IU: LOT: EXP: ADVATE [Antihemophilic Factor (Recombinant)] For Intravenous Administration Only Rx Only ADVATE and diluent preassembled in BAXJECT III system Directions for use: see package insert Product does not contain plasma/albumin 6521260 Takeda IU: LOT: EXP: ADVATE [Antihemophilic Factor (Recombinant)] NDC 0944-3045-10 Single-dose only For Intravenous Administration Only Rx Only Lyophilized powder for reconstitution ADVATE and diluent preassembled in BAXJECT III system Product does not contain plasma/albumin ACTUAL POTENCY 5 mL Takeda ADVATE [Antihemophilic Factor (Recombinant)] NDC 0944-3045-11 For Intravenous Administration Only ADVATE and diluent preassembled in BAXJECT III system Do not open until time of use Do not use if packaging is opened or damaged Discard unused portion

A total of 110 4 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice