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Aging Cell 7(5):717732 Chondrogianni N, Voutetakis K, Kapetanou M, Delitsikou V, Papaevgeniou N, Sakellari M, Lefaki M, Filippopoulou K, Gonos ES (2015) Proteasome activation: an innovative promising approach for delaying aging and retarding age-related diseases

Control and acivicin-treated cells were subjected to heat shock at 45 C for 3 h and then transferred to 37 C for 24 h

The study found that this fusion protein could regulate mitochondria-related functions, reduce the apoptosis of isolated rat islets cultured with pro-inflammatory cytokines, enhance their viability, and maintain islet function

Cell lysates were clarified by centrifugation at 14,000 rpm for 20 min at 4 C and passed through Zeba spin desalting columns (Pierce, 7 K MWCO) to remove excess probe and protein concentration was determined by BCA assay