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Thyroid function tests should be monitored in patients receiving thyroid hormone replacement, and the timing of levothyroxine administration relative to semaglutide injection may need to be optimized to ensure consistent absorption

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Animals This study was conducted with 12-week-old, 200 g body weight, male albino Wistar rats, randomly assigned at 6 rats/group/interval

Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37C

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