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Description
Grano and De Tullio therefore proposed that organisms that have lost vitamin C biosynthesis have an advantage: they can finely regulate HIF1 activation on the basis of the dietary intake of vitamin C (Grano & De Tullio 2007)

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After 15 min agitation at 8 C followed by 15 min centrifugation at 20800 g and 4 C, the supernatant was collected in a 15 ml Falcon tube and protein content was measured using the DC TM Protein Assay (Biorad, Feldkirchen, Germany)

Nat Genet 2000;26:8992

Conflict of Interest: None
