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doi: 10.1056/NEJMoa2002032 25 NishigaMWangDWHanYLewisDBWuJC
Conditioned medium was harvested, filtered through 0.45-m filters, concentrated by ultracentrifugation (81550 g, 2 h at 4 C) and stored at 80 C
Intracavernosal injection therapy has shown high success rates, even among patients who dont find success with oral ED medications
The pelleted cells were washed twice with 0.85% NaCl, resuspended in 1 mL 30% methanol/water, and lysed on ice with ultrasonication
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