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Based on the displayed structures, and in particular with that of the glutathione conjugate of etacrynic acid [132], which is itself a Michael acceptor, it is well conceivable that glutathione acrylate can dock into the glutathione activating pocket of the GST enzymes and irreversibly bind to the essential tyrosine phenolate function, thus inactivating the enzyme

Seminiferous tubules isolated from late pubertal rats showed a response similar to that of seminiferous tubules from adult rats, except that the decreases in incorporation were smaller

64 HemilaH.ChalkerE

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Each experiment was replicated twice