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MSCs were seeded at 2 10 4 cells/ml in 24-well plates (Falcon) in alpha minimum essential medium (MEM) supplemented with 10% FBS, penicillin/streptomycin (1%), L-glutamine (1%) and 0.5 ng/ml basic fibroblast growth factor (bFGF) and incubated overnight to allow cells adhesion

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Cysteine-glycine, a breakdown product of extracellular glutathione (Figure 1E), was also elevated in kidneys with TLS, suggesting extracellular glutathione transport