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Heights of the different zones of chondrocytes were measured parallel to the chondrocyte columns using the MacBAS version 2.5 program (Koshin Graphic Systems, Sapporo, Japan)

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Total RNA was used as the template for cDNA synthesis with Superscript II reverse transcriptase (Invitrogen, CA, USA)

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In ISCs, the regulation of CncC works in the opposite way compared to differentiated cells: CncC activity is inhibited in response to stress in ISCs, whereas it is induced in differentiated cells

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