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Because the aging defect was not relieved by uncoupling, the electron transport chain (ETC), rather than the phosphorylation system, was identified as the site of the defect

Ovcar8-EV, n = 7

Am J Physiol Regul Integr Comp Physiol 2006;290:R793-802

CrAT deficiency suppressed the residual glucose dependent de novo lipogenesis in ACLY KO cells and abrogated the effect of carnitine supplementation in promoting de novo lipogenesis (Figure 6G, Supplemental Figure 7I)