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Then the samples were mounted in a Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies, Germany) for binding measurements, which were performed at 25 C using 20% blue LED excitation power (blue filter, excitation 460480 nm, emission 515530 nm) and 60% IR-laser power with off/on times of 3 s and 20 s

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Based on the above evidence, within the uterine fibroid microenvironment dominated by E 2 and progesterone, OS may exert synergistic effects with hormonal signaling by modulating the activation status of key signaling pathways such as TGF-/Smad3, PI3 K/Akt/mTOR, and MAPK, collectively forming a complex pro-growth signaling network

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