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The PCR was adjusted to initial denaturation 95 C for 2 min, 35 cycles of denaturation at 95 C for 30 s, annealing at 55 C for 30 s, extension at 72 C for 1 min, with a final extension at 72 C for 5 min

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[DOI] [PubMed] [Google Scholar] 253.Liatsopoulou A, Varvaresou A, Mellou F, Protopapa E

Because the GST protein has a strong binding affinity for GSH, beads coated with the compound can be added to the protein mixture

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