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Description
Often yes, with sensible timing

The media was then removed and the formazan crystals that formed during incubation were dissolved in 1 mL DMSO and processed for OD reading

The Reconstitution Protocol: A Step-by-Step Guide Knowing the math is one thing

Schlipf T., Klinikum Winnenden, Department of Psychiatry and Psychotherapy
It looks like severe swelling, redness, and pain at the injection site are known possible reactions