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For differential expression testing, the R package DESeq2 (v.1.30.1) was used to identify differentially expressed genes

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Screening protocols The diagnosis of LS follows a multi-step approach aimed at identifying tumors with MMR deficiencies and confirming the presence of pathogenic genetic mutations

doi: 10.1111/papr.12516

After the PCR reaction, 1 uL of DpnI restriction enzyme was added to the reaction, and the mix was incubated for 2 h at 37 C
