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The possible mechanism of gut microbiota dysbiosis related to CVDs includes its role in increasing intestinal permeability and triggering inflammation via the LPS/TLR4 as well as NLRP3 pathways, thereby ultimately contributing to the development of CVDs [101]

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Evaluation of the lipophilic properties of opioids, amphetamine-like drugs, and metabolites through electrochemical studies at the interface between two immiscible solutions

Immunofluorescence Staining and Quantification PBS-washed cells on coverslips were fixed with 4% paraformaldehyde (15 min, RT) and permeabilized with 0.1% Triton X-100 (5 min, RT)