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Murine astrocytes obtained from embryo (G355-5, ATCC, USA) were cultured in DMEM containing 10% FBS, 2 mM glutamine, and 200 mM streptomycin/penicillin and then maintained at 90%-95% relative humidity in 5% CO2 at 37 C
The initial irrigation was provided at the time of planting, and subsequent irrigations were scheduled based on the soil moisture content monitoring [44]
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10.1016/j.biopha.2021.111661 328 MiyakeS.KimS.SudaW.OshimaK.NakamuraM.MatsuokaT.et al
