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AEX of the the GSH elution was evaluated by direct loading to a POROS 50 HQ packed column, where CVA21 virions flowed through at 75 mM NaCl at pH 8 and residual serum proteins bound (Supplementary Figure S7C)

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WJS carried out some of the original experiments described

Elbers JM, Asscheman H, Seidell JC, Gooren LJ
