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Gel lanes were cut into 210 pieces (fractions) and reduced in-gel with 10 mM DTT, alkylated with 55 mM iodoacetamide, and digested overnight with 17 ng/L sequencing-grade trypsin (Promega) at 37 C using a slightly modified in-gel digestion method 57

The body's ability to detoxify harmful substances efficiently is crucial for maintaining overall health

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3.3 Epigenetic modulation for differentiation and reprogramming Epigenetic modulations are important mechanisms of transcriptional regulation during normal development

The two adjacent clusters of three models each showed comparably low enrichment scores in the mentioned hallmarks, but high scores in hallmarks G2M checkpoints, as well as E2F targets
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