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After conversion, a 5 5 median convolution filter was applied to sharpen the border of the converted phasor plot, decreasing the variance of phasor distribution (Figure 2A), as described in reference ( FIGURE 2 As a reference point, we imaged transgenic worms that express DHS-3::GFP and VIT-2::GFP (Figures 2BD) with high-concentration (5 M) Nile Red vital staining, the worm with low-concentration (50 nM) Nile Red vital staining (Supplementary Figure S3 left), as well as Nile Red in water and lipid solution as an in vitro reference (Supplementary Figure S4A)