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Neuronal viability, mitochondrial membrane potential, mitochondrial morphology, respiratory function, ATP production, and its potential mechanism were assayed by MTT cell proliferation and cytotoxicity assay kit, cell-permeant MitoTracker probes, mitochondrial stress, real-time ATP rate and western blotting

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* Bio-GEE : biotinylated glutathione ethyl ester DTT : 1,4-dithiothreitol ETC : electron transport chain GSH : reduced glutathione GSSG : oxidized glutathione LDH : lactate dehydrogenase LPS : lipopolysaccharide MitoCDNB : Mitochondrially-targeted 1-chloro-2,4-dinitrobenzene NEM : N-ethylmaleimide OGDHC : 2-oxoglutarate dehydrogenase complex PDC : pyruvate dehydrogenase complex PDCE2 : PDC E2 component PDCE3 : PDC E3 component ROS : reactive oxygen species SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis TLR : toll-like receptor

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