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insights into the mechanism of reaction by electrospray ionization mass spectrometry, 1H NMR, and computational techniques

Oxidation kinetics for roGFP2 WT -PfGrx fusion constructs The in vivo oxidation kinetics from fusion constructs between roGFP2 WT and a variety of glutaredoxins and mutants thereof were shown to correlate very well with the rate constants for the oxidative half-reaction from GSSCys assays in vitro, suggesting that the glutathionylation of the glutaredoxin and not the transfer of oxidation to the fused roGFP2 moiety was rate-limiting under the chosen conditions 15,34,35

[DOI] [PMC free article] [PubMed] [Google Scholar] 37.Abraham E, Arcaroli J, Carmody A, Wang H, Tracey KJ

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Jones, Ph.D., professor in the division of pulmonary medicine at Emory University (3)