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For immunofluorescence staining, brain tissues were dissected out, post-fixed by 4% paraformaldehyde (PFA) for 12 h, embedded in paraffin, cut into 5 mm sections and mounted on slides for subsequent staining

Weight loss in cuprizone-fed animals over the first week of administration is thought to be indicative that the drug is efficacious at producing demyelinating pathology 34,40