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RT-PCR The total RNA from M0, M1, and M2 macrophages was extracted using RNeasy Mini Kit (QIAGEN Inc., 74106), and the amount and quality were measured by Nanodrop 2000 (Thermo Scientific)

Healthy adults require an average intake of 4-7 mcg daily to maintain B 12 status.34 Indications for B 12 treatment, administration routes, and preparations Treatment with B 12 may be required for a variety of reasons (table 1)

5a) or their expression is restricted only to the DP ( Rspo3

Brain disorders biomarker detection is mainly hampered by the bloodbrain barrier (which maintains strict and different chemical climates between the brain and the periphery) and difficulty to probe the chemistry, i.e., neurotransmission, of an intact brain in vivo [8,60,90]