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To sterilize the extract and prevent potential damage to the liquid chromatography device during injection, the solution was filtered through a 0.45-m membrane filter 19

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Although this ATP-consuming pathway is energetically unfavourable, this could be related to the continuation of re-dox equilibrium to re-oxidise the excess of NADH produced during glycolysis

However, it is an invasive, often painful procedure with significant cost and downtime

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