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Next, 40 mL of seed culture grown overnight was added to 4 L of LB broth and incubated at 37 C until it reached an optical density of 0.6 as measured at 600 nm ( A 600 )
For example, the S100 calcium-binding protein A9 (S100A9) (Supplmentary Table S2), which plays an important role in the regulation of inflammatory processes and the immune response (Ryckman et al., 2003), was 99.7-fold more highly expressed in the seal compared to the mouse
[PMC free article: PMC1559781] [PubMed: 16891413] 179
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The organism is then more susceptible to harmful external influences
