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K-means clustering analysis of amplicon sequence variants by animal demonstrated that NNMTi-treated DIO mice switched to LD had a distinct microbiome pattern that was highlighted by decreased Erysipelatoclostridium and increased Lactobacillus relative abundances compared to vehicle counterparts

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This exceptional selectivity profile enables researchers to attribute observed effects specifically to NNMT inhibition, rather than off-target activities that plague less specific compounds