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Innov Food Sci Emerg 11:210218 [Google Scholar] Gulcin I (2011) Antioxidant activity of eugenol-a structure and activity relationship study

Oxidative stress, which is tightly intertwined with lipid peroxidation, has also been heavily reported as an integral part of BD pathogenesis

The expression of HSP genes, such as HSP70 , HSP90 , and HSP27 , is time- and tissue-dependent, with acute heat stress inducing rapid upregulation, especially in the heart, liver, and intestines [10]

After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)

This could make the therapeutic decision easier, and patients benefit more from the clinical outcome