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Long-term intervention with lactate and high-intensity interval training (HIIT) in elderly mice showed an increase in the expression of several angiogenesis-associated pathway proteins in the hippocampus, enhancing mitochondrial activity and metabolism-related substances

Mice were single-housed in cages equipped with a running wheel (23 cm in diameter, Techniplast)

Follicular Dendritic Cell Secreted Protein ( FDCSP ) and C-X-C Motif Chemokine Ligand 13 ( CXCL13 ) for GC

Make the standard curve according to the absorbance (x) and concentration (y, g/mL)

The coverslips were first incubated in a blocking solution (5% BSA in PBS, pH 7.4) for 1 h at room temperature, followed by incubation with anti-FPR1 or anti-CXCR1 antibody at 4 C for 24 h