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Improved lyophilization conditions for long-term storage of bacteriophages

[DOI] [PMC free article] [PubMed] [Google Scholar] Seiml-Buchinger V, Reifschneider E, Bittner A, Baier M

Problem: Powder won't dissolve completely Causes: Water too cold Not enough time Damaged peptide Solutions: Let sit for 5-10 minutes undisturbed Gently swirl (don't shake) Warm hands around vial (body heat helps) If still not dissolving after 15 minutes, peptide may be degraded Problem: Solution is cloudy or has particles Causes: Contamination Degraded peptide Improper storage before reconstitution Solutions: Do not use cloudy solution Discard the vial Contact vendor for replacement Never inject cloudy peptides Problem: Can't draw liquid from vial Causes: Vacuum in vial Needle blocked Solutions: Inject air into vial before drawing (equal amount to what you're withdrawing) Make sure needle tip is below liquid level Use fresh needle if current one is blocked Problem: Injection site reactions Causes: Injecting too fast Not rotating sites Contaminated peptide Solutions: Inject slower (10 seconds minimum) Rotate sites minimum 1 inch apart Check for cloudiness before injection If reactions persist, discard vial Problem: Uncertain about concentration Causes: Forgot to label vial Added wrong amount of water Mixed up multiple vials Solutions: Always label immediately after reconstitution Use our peptide reconstitution calculator and write exact concentration on vial When uncertain, discard and start fresh (better safe than wrong dose) Reconstitution for specific peptides Quick reference for common peptides

Approval to market 2% topical solution to women was granted October 1996

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