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The peptide exists physiologically as a complex with copper(II) ions and is found in saliva, urine, and plasma, where its concentration declines significantly with age

9 Importantly, glutathione plays a central role in liver detoxification by helping convert fat-soluble toxins into water-soluble compounds for easier elimination

Achieving a detailed understanding of intracellular GSH homeostasis depends on the development of tools to map GSH compartmentalization and intra-organelle fluctuations

The homogenized tissue was then incubated at 80 C for 15 min and then centrifuged at 18,000 g at 4 C for 5 min

Therefore, in order to investigate the importance of GSH under metal stress, GSH-deficient conditions in mutants due to decreased GSH1 activity are preferred instead of knockout plants [23]