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The culture medium consisted of DMEM (#044-29765, FUJIFILM Wako Pure Chemical Corporation) supplemented with 10% fetal bovine serum (#10270106, Thermo Fisher Scientific) and 1 penicillinstreptomycin solution (#168-23191, FUJIFILM Wako Pure Chemical Corporation)

Then, Mountjoy KG, Robbins LS, Mortrud MT, Cone RD

Clinical trials noted that while it does not induce a full skin tan, localized focal hyperpigmentation (skin darkening) occurs more frequently with prolonged use (up to ~8% in some populations) 5

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Bennet Harry, a blogger, went through a similar experience when they tried to lose weight by following a low-calorie diet plan and exercising regularly along with taking liposuction injections