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Even though all three assays assess cell death or cell viability, they differ fundamentally

Additionally, the production of hydrogen peroxide is encouraged, aggravating the effects of oxidative stress

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The extract and cells were scraped and collected into 2 ml EP tubes, held at 4 C for a further 5 min and centrifuged for 5 min at 12,000 g and solvent in the resulting supernatant was evaporated using a speed-vac

Minimize air exposure by: Using appropriate reconstitution volumes (don't leave excessive headspace) Limiting the number of needle punctures Purging vials with inert gas (nitrogen or argon) if available How long do peptide solutions actually last