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Description
The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode

Poly-ubiquitin chains are particularly important, as they often label target proteins to be sent to the proteasome for degradation, which is a major mechanism by which cells remove unwanted or damaged proteins

Often responds well, but dense or layered ink may need multiple sessions

And the measures of increased ROS levels were associated with increased sensitivity to H 2 O 2 as well as elevated levels of lipid peroxidation and protein tyrosine nitration, a marker of nitro-oxidative damage (337)

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