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The reduced level of ATP will trigger an increase in the rate of mitochondrial oxidative phosphorylation leading to oxidation of the NADH produced during the metabolism of ethanol

However, the three TMD type tags, GST-EGFP-TMD ITB3 , GST-EGFP-TMD ITAV, and GST-EGFP-TMD ITA5 , remained mainly in the cytoplasm (Figure 2B)

For measurements with ATP9HTmGold, cells with appropriate localization of the sensor and segmentation of mitochondria were selected manually from the ratiometric images