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Epistatic control of intrinsic resistance by virulence genes in Listeria

The authors are extremely thankful to Dr Sheena Tiong Yin Xin (Universiti Malaya) who kindly gave her time to provide English language editing service

coli Rosetta (DE3) cells transformed with the vectors pET15b- saci_2032 and pET15b- saci_1118 , respectively, were cultured in terrific broth (TB) supplemented with 150 g mL 1 ampicillin and 25 g mL 1 chloramphenicol at 37 C

The original two-step magnetic bead method designed in our lab gives a reasonable yield (as modified, 40%), is inexpensive, fast (4.5hours) and highly specific, (extracts only ssDNA autoantibodies as visualized by nanoscale sensitive silver staining)
